| 1 |
<html> |
| 2 |
<title>Slime mold QuickLoad data directory</title> |
| 3 |
<body> |
| 4 |
<h2> |
| 5 |
Slime mold QuickLoad data directory |
| 6 |
</h2> |
| 7 |
<p> |
| 8 |
This directory contains annotation files for the slime mold |
| 9 |
Dictyostelium discoideum strain Ax4 genome that was available as of |
| 10 |
May, 2009. Ann Loraine (of the <a |
| 11 |
href="http://www.transvar.org">Loraine Lab</a>) created all the files |
| 12 |
you see here using GFF files obtained in July 2010 from the <a |
| 13 |
href="http://www.dictybase.org/Downloads/"> dictyBase file Download |
| 14 |
section</a>. |
| 15 |
</p> |
| 16 |
<p> |
| 17 |
To access these data in Integrated Genome Browser, launch IGB (see: |
| 18 |
<a href="http://www.bioviz.org/igb">bioviz.org/igb</a>) and |
| 19 |
choose species Dictyostelium discoideum Ax4 and then genome version |
| 20 |
D_discoideum_Ax4_May_2009 in the Data Access Panel. |
| 21 |
(For documentation about IGB, see <a href="http://wiki.transvar.org/confluence/display/igbman/Home">The IGB User's Guide</a>.) |
| 22 |
</p> |
| 23 |
<p> |
| 24 |
All the available data files you see in this directory |
| 25 |
should appear in the Data Sources section of the Data Access panel. If you want to load |
| 26 |
everything all at once into several tracks, choose ALL and then choose Load Mode |
| 27 |
Whole Genome under the Load Mode section of the Data Access Panel. The Dicty genome is |
| 28 |
a very manageable size, and so we highly recommend you load everything all at once! |
| 29 |
</p> |
| 30 |
<p> |
| 31 |
To view genomic sequence, zoom in and click Load Sequence in View or Load All Sequence. The |
| 32 |
latter will load all the sequence for the current chromsome. Since this genome is quite |
| 33 |
small, we highly recommend the Load All Sequence option. |
| 34 |
</p> |
| 35 |
<p> |
| 36 |
To load next-gen sequencing data from sorted, indexed BAM files, just click-drag them |
| 37 |
from your desktop or a server into the IGB interface. The same goes for "bar" (tiling |
| 38 |
array) files. |
| 39 |
</p> |
| 40 |
<p> |
| 41 |
We gratefully acknowledge Siddhartha Basu of Northwestern University and |
| 42 |
<a href="http://www.dictybase.org/About_dictyBase/">the |
| 43 |
dictyBase Team</a> for helping us set up this Dicty QuickLoad site. Thank you |
| 44 |
and we look forward to working with you in future! |
| 45 |
</p> |
| 46 |
<p> |
| 47 |
Sincerely,<br/> |
| 48 |
The Loraine Lab IGB team |
| 49 |
</p> |
| 50 |
</body> |
| 51 |
</html> |