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aloraine |
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<body> |
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<h1>Welcome</h1> |
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<p> |
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The data files listed below are formatted for visualization in the |
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Integrated Genome Browser, available from <a |
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href="https://bioviz.org">BioViz.org</a>. |
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</p> |
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<p> |
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Data files are from <a href="http://www.thellungiella.org">thellungiella.org</a>. Files were downloaded in December 2012 and reformatted for visualization in IGB. |
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</p> |
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<p> |
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For information about the genome sequence and annotations, please |
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contact Maheshi Dassanayake and Dong-Ha Oh. For information about this QuickLoad site or Integrated Genome Browser, contact Ann Loraine. |
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</p> |
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<p> |
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The genome sequence and annotations for Thellungiella parvula (common |
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name Anatolian cress) were published in the journal Nature Genetics |
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in 2011. See: <a href="http://www.ncbi.nlm.nih.gov/pubmed/21822265">The genome of the extremophile crucifer Thellungiella parvula</a>. |
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</p> |
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<h2>About the files</h2> |
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<p> |
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Files with extension .gz were compressed and indexed using bgzip and |
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tabix tools from Heng Li. |
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</p> |
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<p> |
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The BED file T_parvula_May_2012.bed.gz is BED14 (bed detail) format, |
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developed originally in the UCSC Genome Bioinformatics group and described on their site. |
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</p> |
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<p> |
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In IGB, BED14 field 13 is designated |
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the "gene name" field and field 14 is desigated as the description |
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field. Both can be searched using the coordinates box or the Search |
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tab once the file is loaded into IGB. |
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</p> |
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<p> |
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T_parvula_May_2012.bed.gz was created from TpV84_ORFs.gff from |
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thellungiella.org. In this case, BED14 fields 13 and 4 are identical |
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and field 14 contains the Note attribute from the GFF extra feature |
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field. The time stamp of the GFF file we used was Dec. 28, 2011 and |
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the file was downloaded in Dec. 2012. The original file contained |
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transcriptional start site and polyadenylation signals that were not |
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included in the reformatted BED file because the format does not |
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support these feature types except as field 2, which represents the start of |
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transcription for a gene model. Boundaries of CDS, five_prime_UTR, and |
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three_prime_UTR features were used to delimit exon boundaries in the |
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BED output. For information about software used to perform the format |
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conversion, please see log messages associated with this file. |
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</p> |
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<p> |
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The .2bit file contains the genome sequence for parvula assembly |
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version 2, released May, 2012. The fasta file was converted to .2bit |
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format using Jim Kent's faToTwoBit tool. |
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</p> |
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</body> |
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</html> |